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Western Blot (WB) (Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody and the same antibody preincubated with blocking peptide.)

Rabbit Melanoma-associated antigen D1 Polyclonal Antibody | anti-Maged1 antibody

Melanoma-associated antigen D1 Antibody

Gene Names
Maged1; NRAGE; Dlixin; Dlxin1; Dlxin-1; MAGE-D1; mFLJ00163; DXBwg1492e; 2810433C11Rik; 5430405L04Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Synonyms
Melanoma-associated antigen D1; Polyclonal Antibody; Melanoma-associated antigen D1 Antibody; MAGED1; anti-Maged1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total Melanoma-associated antigen D1 protein.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
775
Applicable Applications for anti-Maged1 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:1000
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1.
Target Name
Melanoma-associated antigen D1
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody and the same antibody preincubated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody and the same antibody preincubated with blocking peptide.)
Related Product Information for anti-Maged1 antibody
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.
Product Categories/Family for anti-Maged1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
85,670 Da
NCBI Official Full Name
melanoma-associated antigen D1
NCBI Official Synonym Full Names
melanoma antigen, family D, 1
NCBI Official Symbol
Maged1
NCBI Official Synonym Symbols
NRAGE; Dlixin; Dlxin1; Dlxin-1; MAGE-D1; mFLJ00163; DXBwg1492e; 2810433C11Rik; 5430405L04Rik
NCBI Protein Information
melanoma-associated antigen D1
UniProt Protein Name
Melanoma-associated antigen D1
UniProt Gene Name
Maged1
UniProt Synonym Gene Names
Nrage
UniProt Entry Name
MAGD1_MOUSE

Uniprot Description

NRAGE: a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although NRAGE shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. Binds p75 neurotrophin receptor (p75NTR) and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members.

Protein type: Adaptor/scaffold

Cellular Component: protein complex; membrane; cytoplasm; plasma membrane; chromatin; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; positive regulation of MAP kinase activity; positive regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; regulation of circadian rhythm; negative regulation of transcription, DNA-dependent; negative regulation of epithelial cell proliferation

Research Articles on Maged1

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Product Notes

The Maged1 maged1 (Catalog #AAA9408018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Melanoma-associated antigen D1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Melanoma-associated antigen D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the Maged1 maged1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Melanoma-associated antigen D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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