Western Blot (WB)
(Western blot of PRMT5 antibody. Cytoplasmic extract of Jurkat cells (30 ug per lane) probed with PRMT5 antibody (1:1,000).)
Rabbit anti-Human PRMT5 Polyclonal Antibody | anti-PRMT5 antibody
PRMT5 antibody (pAb)
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
PRMT5; Polyclonal Antibody; PRMT5 antibody (pAb); JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5; anti-PRMT5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.65ug/ul (varies by lot)
Applicable Applications for anti-PRMT5 antibody
Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
WB: 1:500-1:2,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This PRMT5 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human PRMT5.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Western blot of PRMT5 antibody. Cytoplasmic extract of Jurkat cells (30 ug per lane) probed with PRMT5 antibody (1:1,000).)
Western Blot (WB)
(Western blot of PRMT5 antibody. Cytoplasmic extract of Jurkat cells (30 ug per lane) probed with PRMT5 antibody (1:1,000).)
Related Product Information for anti-PRMT5 antibody
Short Description: Our PRMT5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates PIWI proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates PIWI proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Product Categories/Family for anti-PRMT5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,311 Da
NCBI Official Full Name
protein arginine N-methyltransferase 5 isoform a
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; histone-arginine N-methyltransferase PRMT5; jak-binding protein 1; shk1 kinase-binding protein 1 homolog
UniProt Protein Name
Protein arginine N-methyltransferase 5
Protein Family
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
Similar Products
Product Notes
The PRMT5 prmt5 (Catalog #AAA388716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRMT5 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT5 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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