Rabbit CARD11 Polyclonal Antibody | anti-CARD11 antibody

Anti-CARD11 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102514

• Gene Names: Card11; BIMP3; CARMA1; 0610008L17Rik; 2410011D02Rik
• Reactivity: Mouse; Predicted to React with: Human (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: CARD11; Polyclonal Antibody; Anti-CARD11 Antibody; Rabbit Polyclonal; CARD11 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-CARD11 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CARD11
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CARD11 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:200-1:1000; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse CARD11
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse CARD11, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Card11 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of Card11 in mouse spleen cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Mouse Card11 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane and cytoplasm.)

Related Product Information for anti-CARD11 antibody

The aggressive activated B cell-like subtype of diffuse large B-cell lymphoma is characterized by aberrant B-cell receptor (BCR) signaling and constitutive nuclear factor kappa-B (NF-kappaB) activation, which is required for tumor cell survival. BCR-induced NF-kappaB activation requires caspase recruitment domain-containing protein 11 (CARD11), and CARD11 gain-of-function mutations are recurrently detected in human diffuse large B-cell lymphoma (DLBCL). Enforced activation of CARD11/BCL10/MALT1 signaling is sufficient to drive transformed B-cell expansion in vivo and identify the JNK pathway as a therapeutic target for ABC-DLBCL. The scaffold protein caspase recruitment domain-containing protein 11 (CARD11) is implicated in the regulation of inflammation and autoimmunity. CARD11 is involved in the pathogenesis of CIA by formation of the CARD11/Bcl10 complex and enhancement of the Th17 cell response. Targeting CARD11 provides a novel research direction in the development of therapeutic strategies for RA. CARD11 is a multidomain adapter that is required for TCR signaling to the IkappaB kinase (IKK) complex. During TCR signaling, the region in CARD11 between the coiled-coil and PDZ domains is phosphorylated by protein kinase Ctheta (PKCtheta) in a required step in NF-kappaB activation. CARD protein 11 (CARD11) and CARD protein 14 (CARD14) are novel CARD-containing proteins that belong to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 161086929
• NCBI GeneID: 108723
• NCBI Accession #: NP_780571.2
• NCBI GenBank Nucleotide #: NM_175362.2
• UniProt Accession #: Q8CIS0; Q4VA14; Q6KAS3; Q8BYV0
• Molecular Weight: 133,468 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 11
• NCBI Official Symbol: Card11
• NCBI Official Synonym Symbols: BIMP3; CARMA1; 0610008L17Rik; 2410011D02Rik
• NCBI Protein Information: caspase recruitment domain-containing protein 11
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: Card11

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5|5 G2

Cellular Component: CBM complex; cytoplasm; extracellular exosome; immunological synapse; membrane; membrane raft; plasma membrane; T cell receptor complex

Molecular Function: CARD domain binding; protein binding

Biological Process: B cell differentiation; B cell proliferation; homeostasis of number of cells; I-kappaB kinase/NF-kappaB signaling; immunoglobulin production; interleukin-2 secretion; lymphocyte activation; positive regulation of B cell proliferation; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-2 biosynthetic process; positive regulation of NF-kappaB transcription factor activity; positive regulation of T cell activation; positive regulation of T cell proliferation; regulation of apoptosis; regulation of B cell differentiation; regulation of immune response; regulation of T cell differentiation; signal transduction; T cell activation; T cell costimulation; thymic T cell selection; TORC1 signaling