Rabbit Formin Binding Protein 1-like Polyclonal Antibody | anti-FNBP1L antibody

Formin Binding Protein 1-like, NT (C1orf39, FLJ20275, TOCA1, Toca-1, Transducer of Cdc42-dependent actin assembly protein 1)

Cat. Num. AAA619837

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Formin Binding Protein 1-like; Polyclonal Antibody; NT (C1orf39; FLJ20275; TOCA1; Toca-1; Transducer of Cdc42-dependent actin assembly protein 1); Anti -Formin Binding Protein 1-like; anti-FNBP1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TOCA-1. Species crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-FNBP1L antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic peptide corresponding to 15aa from near the amino terminus of the human TOCA-1 (GenBank accession no. NP_001020119).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 ug/ml.)

Related Product Information for anti-FNBP1L antibody

Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N- WASP and Cdc42 and is essential for Cdc42- and PIP2- induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex (1). Decreased expression of TOCA- 1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation (2). It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain (2). Multiple isoforms of TOCA-1 are known to exist.

Product Categories/Family for anti-FNBP1L antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 56205711
• UniProt Accession #: Q5T0N5; Q5T0N6; Q6B097; Q6P653; Q6R4Q4; Q9NXG1
• Molecular Weight: 69,977 Da
• NCBI Official Full Name: formin binding protein 1-like
• UniProt Protein Name: Formin-binding protein 1-like
• Protein Family: Formin-binding protein
• UniProt Gene Name: FNBP1L
• UniProt Synonym Gene Names: C1orf39; TOCA1
• UniProt Entry Name: FBP1L_HUMAN

Uniprot Description

FNBP1L: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens. Belongs to the FNBP1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cell cortex

Molecular Function: protein binding; lipid binding

Biological Process: positive regulation of filopodium formation; vesicle transport along actin filament; vesicle organization and biogenesis; membrane invagination; autophagy; cilium biogenesis; membrane budding