Rabbit anti-Human, Rat CARD11 Polyclonal Antibody | anti-CARD11 antibody

CARD11 (M12) Polyclonal Antibody

Cat. Num. AAA3002320

• Gene Names: CARD11; PPBL; BENTA; BIMP3; IMD11; CARMA1; IMD11A
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CARD11; Polyclonal Antibody; CARD11 (M12) Polyclonal Antibody; Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; Carma 1; CARMA1; anti-CARD11 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CARD11 polyclonal antibody detects endogenous levels of CARD11 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1154

• Applicable Applications for anti-CARD11 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Recombinant full length Human CARD11.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Carma 1 (M12) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:The Thymus tissue lysate of Rat (30ug) Lane3:Myla2059 whole cell lysate (40ug))

Related Product Information for anti-CARD11 antibody

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

NCBI and Uniprot Product Information

• NCBI GI #: 172046231
• NCBI GeneID: 84433
• UniProt Accession #: Q9BXL7
• Molecular Weight: ~ 133kDa
• NCBI Official Full Name: Caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family member 11
• NCBI Official Symbol: CARD11
• NCBI Official Synonym Symbols: PPBL; BENTA; BIMP3; IMD11; CARMA1; IMD11A
• NCBI Protein Information: caspase recruitment domain-containing protein 11
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD11
• UniProt Synonym Gene Names: CARMA1; Carma 1
• UniProt Entry Name: CAR11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft

Molecular Function: guanylate kinase activity; protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation

Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy