Rabbit CARD11 Polyclonal Antibody | anti-CARD11 antibody

Phospho-CARD11 (Ser652) Antibody

Cat. Num. AAA9613290

• Gene Names: CARD11; PPBL; BENTA; BIMP3; IMD11; CARMA1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: CARD11; Polyclonal Antibody; Phospho-CARD11 (Ser652) Antibody; 0610008L17Rik; 2410011D02Rik; Bcl10 interacting maguk protein 3; BIMP 3; BIMP3; CAR11_HUMAN; CARD 11; CARD containing MAGUK protein 3; Card maguk protein 1; CARD-containing MAGUK protein 1; CARD11 protein; Carma 1; CARMA1; Caspase recruitment domain containing protein 11; Caspase recruitment domain family member 11; Caspase recruitment domain-containing protein 11; MGC133069; anti-CARD11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 only when phosphorylated at Ser652.; Tissue Specificity: Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CARD11 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human CARD11 around the phosphorylation site of Ser652.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
• Subunit Structure: Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail). Interacts with BCL10; as part of a CBM (CARD11-BCL10-MALT1) complex involved in NF-kappa-B activation.
• Subcellular Location: Cytoplasm. Membrane raft.; Note: Colocalized with DPP4 in membrane rafts.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells(LPS 4h treatment), using Phospho-CARD11 (Ser652) Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Jurkat  , using Phospho-CARD11 (Ser652) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-CARD11 antibody

Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 172046231
• NCBI GeneID: 84433
• UniProt Accession #: Q9BXL7; Q2NKN7; Q548H3; A4D1Z7
• Molecular Weight: 133,284 Da
• NCBI Official Full Name: Caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 11
• NCBI Official Symbol: CARD11
• NCBI Official Synonym Symbols: PPBL; BENTA; BIMP3; IMD11; CARMA1
• NCBI Protein Information: caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD11
• UniProt Synonym Gene Names: CARMA1; Carma 1
• UniProt Entry Name: CAR11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft

Molecular Function: guanylate kinase activity; protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation

Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy