Rabbit anti-Human Calumenin Polyclonal Antibody | anti-CALU antibody

Anti-Calumenin Antibody, Rabbit Polyclonal

Cat. Num. AAA8119057

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Calumenin; Polyclonal Antibody; Anti-Calumenin Antibody; Rabbit Polyclonal; Calumenin/CALU Antibody; Rabbit PAb; Antigen Affinity Purified; calumenin; anti-CALU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Calumenin/CALU
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-CALU antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: Recombinant Human Calumenin/CALU Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Calumenin/CALU (rh Calumenin/CALU; O43852-1; Met1-Arg311). Calumenin/CALU specific IgG was purified by Human Calumenin/CALU affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-Calumenin rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:45 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of Calumenin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- Calumenin polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(CALU was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate Lane C:0.5 mg K562 Whole Cell Lysate 4 uL anti- CALU rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- CALU rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50kDa Observed band size :50 kDa)

Related Product Information for anti-CALU antibody

Background: Calumenin belongs to the CREC family. It contains 6 EF-hand domains. Calumenin is expressed in skeletal muscle (at protein level). Calumenin interacts with GGCX and RYR1 in the presence of calcium ions, but not in the presence of EDTA. Calumenin is Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. It seems to inhibit gamma-carboxylase GGCX. Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum.

References

Hartley JL, et al. (2001) DNA cloning using in vitro site-specific recombination. Genome Res. 10 (11):1788-95.Vorum H, et al. (2000) Calumenin interacts with serum amyloid P component. FEBS Lett. 465 (2-3):129-34.Vorum H, et al. (1999) n calumenin localizes to the secretory pathway and is secreted to the medium. Exp Cell Res. 248(2):473-81.