Rabbit anti-Mouse, Rat C5/C5a Polyclonal Antibody | anti-C5 antibody

Anti-C5/C5a antibody

Cat. Num. AAA176937

• Gene Names: C5; Hc; C5a; RGD1561905
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: C5/C5a; Polyclonal Antibody; Anti-C5/C5a antibody; Complement C5; complement component 5; Anaphylatoxin C5a analog antibody; Anaphylatoxin C5a antibody; C5 antibody; C5a antibody; Complement C5 antibody; Complement component C5 antibody; Complement component C5a antibody; CPAMD 4 antibody; CPAMD4 antibody; anti-C5 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 77

• Applicable Applications for anti-C5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot (WB); Concentration: 0.1-0.5ug/ml; Tested Species: Ms, Rat; ; Immunohistochemistry-Paraffin-embedded Section (IHC-P); Concentration: 0.5-1ug/ml; Tested Species: MS, Rat; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 minutes is required for the staining of fomalin/paraffin sections.; ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a (1-18aa DLQLLHQKVEEQAAKYKH), different from the related mouse sequence by four amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20°C. After reconstitution, store at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-C5/C5a antibody, MBS176937, IHC(P)IHC(P): Mouse Spleen Tissue )

Immunohistochemistry (IHC)

(Anti-C5/C5a antibody, MBS176937, IHC(P)IHC(P): Rat Liver Tissue )

Immunohistochemistry (IHC)

(Anti-C5/C5a antibody, MBS176937, IHC(P)IHC(P): Rat Spleen Tissue )

Western Blot (WB)

(Anti-C5/C5a antibody, MBS176937, All Western blottingAll lanes: Anti-C5a(MBS176937) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 40ugLane 2: Mouse Liver Tissue Lysate at 40ugLane 3: NIH Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: PC12 Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 115KD )

Related Product Information for anti-C5 antibody

Description: Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Mouse, Rat.

Background: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 46397770
• NCBI GeneID: 362119
• UniProt Accession #: P08650; Q63078
• NCBI Official Full Name: Complement C5
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: Hc; C5a; RGD1561905
• NCBI Protein Information: complement C5; anaphylatoxin
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Entry Name: CO5_RAT

NCBI Description

likely component of the classical pathway of the complement cascade; may be involved with tissue injury associated with ischemia or sepsis; may be a component of the membrane attack complex [RGD, Feb 2006]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: C5a anaphylatoxin chemotactic receptor binding

Biological Process: cellular calcium ion homeostasis; positive regulation of angiogenesis; positive regulation of chemotaxis; in utero embryonic development; complement activation, alternative pathway; glucose homeostasis; chemotaxis; inflammatory response; negative regulation of dopamine secretion; complement activation, classical pathway; leukocyte migration during inflammatory response