Rabbit SREBP-1 Polyclonal Antibody | anti-SREBF1 antibody

SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)

Cat. Num. AAA622195

• Gene Names: SREBF1; SREBP1; bHLHd1; SREBP-1c
• Reactivity: Bovine, Hamster, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Synonyms: SREBP-1; Polyclonal Antibody; SREBP-1 (Sterol Regulatory Element Binding Protein-1; SREBP1; ADD1; D630008H06; Sterol Regulatory Element Binding Transcription Factor 1; SREBF1); Anti -SREBP-1 (Sterol Regulatory Element Binding Protein-1; anti-SREBF1 antibody

• Host: Rabbit
• Reactivity: Bovine, Hamster, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human SREPB-1. Species Crossreactivity: mouse, rat, and hamster. Predicted to react with porcine based on 100% sequence homology. Immunogen sequence has 94% homology to bovine, 88% homology to Zebrafish and 70% homology to chicken.
• Purity/Purification: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, 150mM NaCl, pH 7.2, 0.05% sodium azide.

• Applicable Applications for anti-SREBF1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: ~65kD (mature form)
• Immunogen: A synthetic peptide made to a portion of the human SREBP1 protein sequence (between residues 700-800). 100% homology with mouse, rat, and hamster proteins. 70% homology with chicken protein.
• Cellular Localization: Integral membrane protein that moves from ER to Golgi in absence of sterols.
• Positive Control: Human liver lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(SREBP-1 Western Blot: ~65kD (mature form))

Related Product Information for anti-SREBF1 antibody

SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. SREBPs may also play some role in the apoptotic processes. SREBP1 is an ~122kD integral membrane protein that moves from ER to golgi for processing. The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi. This is the ~65kD mature form that is detected by this antibody. The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65kD protein

Product Categories/Family for anti-SREBF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 52630419
• NCBI GeneID: 6720
• NCBI Accession #: NP_001005291.1
• NCBI GenBank Nucleotide #: NM_001005291.2
• UniProt Accession #: P36956; Q16062; Q59F52; Q6P4R7; Q6PFW7; Q6PJ36; Q8TAK9; D3DXC4
• Molecular Weight: 121,675 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 1 isoform a
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: SREBF1
• NCBI Official Synonym Symbols: SREBP1; bHLHd1; SREBP-1c
• NCBI Protein Information: sterol regulatory element-binding protein 1; SREBP-1; OTTHUMP00000065589; OTTHUMP00000065590; OTTHUMP00000065591; OTTHUMP00000065592; OTTHUMP00000065593; class D basic helix-loop-helix protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: SREBF1
• UniProt Synonym Gene Names: BHLHD1; SREBP1
• UniProt Entry Name: SRBP1_HUMAN

NCBI Description

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Membrane protein, multi-pass; Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cytoplasm; integral to membrane; nuclear envelope; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: circadian rhythm; response to glucagon stimulus; response to cAMP; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of insulin secretion; positive regulation of histone deacetylation; regulation of fatty acid metabolic process; response to glucose stimulus; aging; response to drug; response to food; cholesterol metabolic process; response to retinoic acid; transcription, DNA-dependent; cellular response to starvation; regulation of transcription from RNA polymerase II promoter; chemical signal regulation of heart rate; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; response to progesterone stimulus; lung development