Rabbit anti-Mouse. c-Met Polyclonal Antibody | anti-MET antibody

c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Cat. Num. AAA629762

• Gene Names: MET; c-Met
• Reactivity: Mouse.
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: c-Met; Polyclonal Antibody; phosphorylated (Tyr1234; Tyr1235) (Hepatocyte Growth Factor Receptor; Scatter Factor Receptor; HGFR; SFR); Anti -c-Met; anti-MET antibody

• Host: Rabbit
• Reactivity: Mouse.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects human c-MET phosphorylated at Y1234/Y1235.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution containing PBS, pH 7.4, 5% trehalose. Reconstitute with 250 ul sterile PBS.
• Concentration: ~0.2 mg/mL(after reconstitution) (varies by lot)

• Applicable Applications for anti-MET antibody: Flow Cytometry, Immunohistochemistry,Western Blot.; ; Other applications not tested.
• Application Notes: Flow Cytometry: 2.5ug/1x10e6 cells using MCF-7 human breast cancer cell line treated with pervanadate, fixed with paraformaldehyde and permealized with methanol. ; ; Immunohistochemistry: 5-15ug/ml using immersion fixed, frozen section of mouse embryo.; ; Western Blot: 0.5ug/ml using pervanadate-treated MDA-MB-468 human breast cancer cell line .; ; Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Flow Cytometry: MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 100uM pervanadate for 10 mins. (dark orange filled histogram), then stained with MBS629762 or control antibody (blue open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody. To facilitate intracellular staining, cells with fixed with paraformaldehyde and permeabilized with methanol.)

Immunohistochemistry (IHC)

(Immunhistochemical staining of immersion fixed, frozen sections of mouse embryo (13 d.p.c) using MBS629762 at 15ug/ml (overnight incubation at 4°C. Tissue was stained using an anti-rabbit HRP-DAB staining kit and counterstained with hematoxylin (blue).)

Western Blot (WB)

(Western blot shows immunoprecipitate of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100uM pervanadate for 10 mins. PVDF membrane was probed with 0.5ug/ml MBS629762 , followed by HRP-conjugated anti-rabbit IgG secondary antibody. A specific band was detected for phospho HGF R/cMet (Y1234/Y1235) at ~145kD. Experiment was conducted under reducing conditions.)

Related Product Information for anti-MET antibody

Hepatocyte growth factor receptor (HGF R), also known as c-MET, is a receptor tyrosine kinase that consists of an extracellular chain and a longer chain. The chain contains the transmembrane helix and the cytoplasmic portion. Ligand-stimulated phosphorylation of tyrosine 1234 and tyrosine 1235 in HGF R's chain results in stimulation of intrinsic kinase activity.

Product Categories/Family for anti-MET antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 25815188
• NCBI GeneID: 403438
• UniProt Accession #: Q75ZY9; A0M8U9
• Molecular Weight: 154,637 Da
• NCBI Official Full Name: c-Met
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: C-methyltransferase
• UniProt Gene Name: MET
• UniProt Entry Name: MET_CANFA

Uniprot Description

Function: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10

Subcellular location: Membrane; Single-pass type I membrane protein

Domain: The kinase domain is involved in SPSB1 binding

Post-translational modification: Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.