Integrin alpha 4 Recombinant Protein | ITGA4 recombinant protein

Integrin alpha 4 Recombinant Protein

Cat. Num. AAA153305

• Gene Names: ITGA4; IA4; CD49D
• Applications: ELISA, Western Blot
• Purity: ~95%
• Synonyms: Integrin alpha 4; Integrin alpha 4 Recombinant Protein; IA4; CD49D; Integrin alpha-4; CD49 antigen-like family member D; integrin; alpha 4 (antigen CD49D; alpha 4 subunit of VLA-4 receptor); ITGA4 recombinant protein

• Purity/Purification: ~95%
• Form/Format: Liquid
• Sequence: aa 131 - 275
• Sequence Length: 1032

• Applicable Applications for ITGA4 recombinant protein: ELISA (EIA), Western Blot (WB)
• Application Notes: This recombinant protein can be used for WB,and ELISA.For research use only.
• Source: E Coli
• Buffer: 50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
• Fusion Tag: Fusion Partner: N-terminal His-tag
• Preparation and Storage: Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.; Shipping: Dry Ice.

SDS-PAGE

(SDS-PAGE analysis of recombinant Integrin alpha 4 fragment on Coomassie Blue-stained 4-20% gradient gel.)

Related Product Information for ITGA4 recombinant protein

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1). Alpha 4(4) chain associates with either beta 1(1) or beta 7 7) chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4). Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells 5). This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4(IN1) PSI 4827) and Integrin 4(IN2)(PSI 4837), but not Integrin 4(CT)(PSI 4783).

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876
• NCBI GenBank Nucleotide #: NM_000885.4
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4; AAB25486
• Molecular Weight: 17 kDa (Calculated)
• NCBI Official Full Name: integrin alpha-4
• NCBI Official Synonym Full Names: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit
• UniProt Protein Name: Integrin alpha-4
• Protein Family: Integrin
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Receptor, misc.; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; metal ion binding; fibronectin binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; heart development; cell-matrix adhesion; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; leukocyte tethering or rolling; blood coagulation; leukocyte migration