Rat AQP-3 ELISA Kit | AQP-3 elisa kit

Rat AQP-3 (Aquaporin 3) ELISA Kit

Cat. Num. AAA2509381

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Rat
• Synonyms: AQP-3; Rat AQP-3 (Aquaporin 3) ELISA Kit; AQP-3 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat AQP-3. No significant cross-reactivity or interference between Rat AQP-3 and analogues was observed.
• Sequence Length: 292

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat AQP-3 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-3 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AQP-3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AQP-3. You can calculate the concentration of AQP-3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4826645
• NCBI GeneID: 360
• NCBI Accession #: NP_004916.1
• NCBI GenBank Nucleotide #: NM_004925.4
• UniProt Accession #: Q92482; O00108; Q6FGT2; Q6FGW6; A8K843; B2RE16; D3DRL3
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: aquaporin-3
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
• UniProt Protein Name: Aquaporin-3
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3
• UniProt Entry Name: AQP3_HUMAN

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Uniprot Description

AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, aquaporin family; Channel, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction

Molecular Function: transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: odontogenesis; positive regulation of immune system process; glycerol transport; response to retinoic acid; cellular water homeostasis; response to vitamin D; transport; water transport; renal water homeostasis; response to calcium ion; excretion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group