Western Blot (WB)
(Western blot analysis of BRSK2 (Phospho-Thr260) using EGF treated RAW264.7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit BRSK2 Polyclonal Antibody | anti-BRSK2 antibody
Phospho-BRSK2 (Thr260) Antibody
Gene Names
BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Dog (100%), Chicken (83%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
BRSK2; Polyclonal Antibody; Phospho-BRSK2 (Thr260) Antibody; anti-BRSK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Dog (100%), Chicken (83%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Dog (100%), Chicken (83%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-BRSK2 (Thr260) Antibody detects endogenous levels of BRSK2 only when phosphorylated at Thr260.
Tissue Specificity: Detected in pancreas islets (at protein level).
Tissue Specificity: Detected in pancreas islets (at protein level).
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BRSK2 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human BRSK2 around the phosphorylation site of Thr260.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated at Thr-174 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-174 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-174 by PP2C. Phosphorylated at Thr-260 by PKA. Phosphorylation at Thr-260 by PKA was not observed in another study, but this may reflect differences in the experimental approach. Phosphorylation at Thr-260 seems to play a role in the regulation of insulin secretion.Polyubiquitinated by the APC complex in conjunction with FZR1, leading to its proteasomal degradation. Targeted for proteasomal degradation by interaction with COPS5. BRSK2 levels change during the cell cycle. BRSK2 levels are low at the G1/S boundary and gradually increase as cells progress into G2 phase. BRSK2 levels decrease rapidly at the end of mitosis.
Subunit Structure
Interacts with FZR1, a regulatory subunit of the APC ubiquitin ligase complex. Interacts with COPS5. Interacts with PAK1.
Similarity
The KEN box motif is required for interaction with FZR1/CDH1 and essential for APC (CDH1)-mediated ubiquitination.Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Subcellular Location
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Perinuclear region. Endoplasmic reticulum.
Note: Detected at centrosomes during mitosis. Localizes to the endoplasmic reticulum in response to stress caused by tunicamycin.
Note: Detected at centrosomes during mitosis. Localizes to the endoplasmic reticulum in response to stress caused by tunicamycin.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of BRSK2 (Phospho-Thr260) using EGF treated RAW264.7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of BRSK2 (Phospho-Thr260) using EGF treated RAW264.7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-BRSK2 antibody
Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion, BRSK2 phosphorylated at Thr-260 can promote insulin secretion. Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,740 Da
NCBI Official Full Name
Serine/threonine-protein kinase BRSK2
NCBI Official Synonym Full Names
BR serine/threonine kinase 2
NCBI Official Symbol
BRSK2
NCBI Official Synonym Symbols
SAD1; SADA; STK29; PEN11B; C11orf7
NCBI Protein Information
serine/threonine-protein kinase BRSK2; protein kinase SAD1B; brain-selective kinase 2; serine/threonine-protein kinase 29; BR serine/threonine-protein kinase 2; serine/threonine-protein kinase SAD-A; brain-specific serine/threonine-protein kinase 2
UniProt Protein Name
Serine/threonine-protein kinase BRSK2
Protein Family
UniProt Gene Name
BRSK2
UniProt Synonym Gene Names
C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
UniProt Entry Name
BRSK2_HUMAN
Uniprot Description
BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.
Protein type: EC 2.7.11.1; EC 2.7.11.26; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; BRSK subfamily
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: centrosome; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm
Molecular Function: protein serine/threonine kinase activity; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding
Biological Process: neuron differentiation; mitosis; peptidyl-serine phosphorylation; exocytosis; axonogenesis; cell division; establishment of cell polarity; actin cytoskeleton reorganization; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation
Research Articles on BRSK2
Similar Products
Product Notes
The BRSK2 brsk2 (Catalog #AAA9614136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-BRSK2 (Thr260) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Dog (100%), Chicken (83%) and may cross-react with other species as described in the data sheet. AAA Biotech's BRSK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the BRSK2 brsk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRSK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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