Rabbit anti-Human, Mouse BRSK2 Polyclonal Antibody | anti-BRSK2 antibody

BRSK2 Antibody

Cat. Num. AAA9404475

• Gene Names: BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: BRSK2; Polyclonal Antibody; BRSK2 Antibody; BR serine-threonine kinase 2; serine-threonine protein kinase 29; STK29 PEN11B; SAD1B kinase; SAD-A; anti-BRSK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 736

• Applicable Applications for anti-BRSK2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 23 amino acid peptide from near the carboxy terminus of human BRSK2.
• Target Name: BRSK2
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 ug/mL.)

Related Product Information for anti-BRSK2 antibody

BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.

Product Categories/Family for anti-BRSK2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 375298744
• NCBI GeneID: 9024
• NCBI Accession #: NP_001243556.1
• NCBI GenBank Nucleotide #: NM_001256627.1
• UniProt Accession #: Q8IWQ3; O60843; O95099; Q5J5B4; Q6ZMQ4; Q8TB60; B3KVE9; E9PLM7
• Molecular Weight: 68,740 Da
• NCBI Official Full Name: serine/threonine-protein kinase BRSK2 isoform 1
• NCBI Official Synonym Full Names: BR serine/threonine kinase 2
• NCBI Official Symbol: BRSK2
• NCBI Official Synonym Symbols: SAD1; SADA; STK29; PEN11B; C11orf7
• NCBI Protein Information: serine/threonine-protein kinase BRSK2
• UniProt Protein Name: Serine/threonine-protein kinase BRSK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: BRSK2
• UniProt Synonym Gene Names: C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
• UniProt Entry Name: BRSK2_HUMAN

Uniprot Description

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.26; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: centrosome; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm

Molecular Function: protein serine/threonine kinase activity; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; mitosis; peptidyl-serine phosphorylation; exocytosis; axonogenesis; establishment of cell polarity; cell division; actin cytoskeleton reorganization; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation