Western Blot (WB)
(Figure 1: Western blot analysis using FRK mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FRK cDNA (2).)
Mouse anti-Human FRK Monoclonal Antibody | anti-FRK antibody
FRK Antibody
Gene Names
FRK; GTK; RAK; PTK5
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
FRK; Monoclonal Antibody; FRK Antibody; Beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; mouse; homolog of; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; PTK5 protein tyrosine kinase 5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK; anti-FRK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
FRK antibody detects endogenous levels of total FRK
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
505
Applicable Applications for anti-FRK antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:100-1:500
Immunogen
Purified recombinant fragment of human FRK expressed in E. Coli
Subcellular Location
Cytoplasm.
Tissue Specificity
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using FRK mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FRK cDNA (2).)
Western Blot (WB)
(Figure 1: Western blot analysis using FRK mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FRK cDNA (2).)
Related Product Information for anti-FRK antibody
Description: The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth.
Function: Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Subunit Structure: Interacts (via the SH3-domain) with PTEN. Interacts with RB1.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Function: Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Subunit Structure: Interacts (via the SH3-domain) with PTEN. Interacts with RB1.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 56 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
tyrosine-protein kinase FRK
NCBI Official Synonym Full Names
fyn related Src family tyrosine kinase
NCBI Official Symbol
FRK
NCBI Official Synonym Symbols
GTK; RAK; PTK5
NCBI Protein Information
tyrosine-protein kinase FRK
UniProt Protein Name
Tyrosine-protein kinase FRK
Protein Family
UniProt Gene Name
FRK
UniProt Synonym Gene Names
PTK5; RAK
NCBI Description
The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]
Uniprot Description
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Research Articles on FRK
Similar Products
Product Notes
The FRK frk (Catalog #AAA9602406) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FRK Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FRK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the FRK frk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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