Rabbit anti-Human HSF1 Polyclonal Antibody | anti-HSF1 antibody

Anti-HSF1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106324

• Gene Names: HSF1; HSTF1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: HSF1; Polyclonal Antibody; Anti-HSF1 Antibody; Rabbit Polyclonal; HSF1 Antibody; Rabbit PAb; Antigen Affinity Purified; heat shock transcription factor 1; Anti-HSTF1 Antibody; anti-HSF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HSF1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HSF1 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human HSF1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human HSF1 (rh HSF1). HSF1 specific IgG was purified by Human HSF1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human HSF1 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human HSF1 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-HSF1 antibody

Heat shock factor protein 1, also known as heat shock transcription factor 1, HSF1, and HSTF1, is a cytoplasm and nucleus protein that belongs to the HSF family. HSF1 is the major transcription factor of HSPs (heat shock proteins) in response to various stresses. Wild type HSF1 (heat shock transcriptional factor 1) is normally inactive. HSF1/HSTF1 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF1/HSTF1 protects cells and organisms against various types of stress, either by triggering a complex response that promotes cell survival or by triggering cell death when stress-induced alterations cannot be rescued. HSF1/HSTF1 is the key protein in regulating the stress response. It can be activated under heat, oxidative, or other stress conditions. Dominant-positive and dominant-negative HSF1 are two types of HSF1 mutants. Both of them gain DNA binding activity in the absence of stress. Also, dominant-positive HSF1 acquires transcriptional activity, which dominant-negative HSF1 does not acquire. HSF1/HSTF1 was also reported to contribute to cell resistance against genotoxic stress, such as that caused by doxorubicin, an anticancer drug in common clinical use.

References

Holmberg, C.I. et al., 2000, Cell Stress Chaperones.5 (3):219-28. Huang, Y.H. et al., 2007, Sheng Wu Gong Cheng Xue Bao. 23 (6): 971-5. Salmand, P.A. et al., 2008, Biol Reprod 79 (6): 1092-101. Lee, Y.J. et al., 2008, Cancer Res 68 (18): 7550-60. Hou, Y. et al., 2009, Mol Biol Rep. 36 (8): 2271-7.

NCBI and Uniprot Product Information

• NCBI GI #: 5031767
• NCBI GeneID: 3297
• NCBI Accession #: NP_005517.1
• NCBI GenBank Nucleotide #: NM_005526.2
• UniProt Accession #: Q00613; Q53XT4; A8K4L0; A8MW26
• Molecular Weight: 57,260 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock transcription factor 1
• NCBI Official Symbol: HSF1
• NCBI Official Synonym Symbols: HSTF1
• NCBI Protein Information: heat shock factor protein 1; HSF 1; HSTF 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSTF1; HSF 1; HSTF 1
• UniProt Entry Name: HSF1_HUMAN

NCBI Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: negative regulation of cell proliferation; embryonic placenta development; embryonic process involved in female pregnancy; mRNA transcription; female meiosis; defense response; spermatogenesis; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation