Immunohistochemistry (IHC)
(Fig: 1 Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using at 1: 2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.)
Rabbit Bag-1 Polyclonal Antibody | anti-bag-1 antibody
Polyclonal Antibody to Bag-1
Reactivity
Cow, Dog, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry
Purity
Serum
Synonyms
Bag-1; Polyclonal Antibody; Polyclonal Antibody to Bag-1; bag-1; F57B10.11; BAG family molecular chaperone regulator 1; Antibody to Bag-1; Anti-Bag-1 Antibody; Bag-1 Antibody; anti-bag-1 antibody
Host
Rabbit
Reactivity
Cow, Dog, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
210
Applicable Applications for anti-bag-1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1: 1000-1: 2000
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
IF/ICC: 1: 500-1: 2000
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
IF/ICC: 1: 500-1: 2000
Immunogen Information
A synthetic peptide of Bag-1 protein (amino acids 205-219 QETERLQSTNLALAE) was used as the immunogen for this antibody
Antigen
Bag-1
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Immunohistochemistry (IHC)
(Fig: 1 Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using at 1: 2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.)
Immunohistochemistry (IHC)
(Fig: 1 Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using at 1: 2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using at 1: 2000. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using at 1: 2000. Hematoxylin-eosin counterstain.)
Related Product Information for anti-bag-1 antibody
This antibody recognizes both BAG-1 and BAG-1 isoforms which contain the peptide immunogen sequence, including BAG-1L. Mouse BAG-1 is a 219 amino acid (aa) protein. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis, ), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis. BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells.
Product Categories/Family for anti-bag-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,010 Da
NCBI Official Full Name
BAG family molecular chaperone regulator 1
NCBI Official Symbol
bag-1
NCBI Protein Information
BAG family molecular chaperone regulator 1
UniProt Protein Name
BAG family molecular chaperone regulator 1
Protein Family
UniProt Gene Name
bag-1
Uniprot Description
May inhibit the chaperone activity of HSP70/HSC70 by promoting substrate release in an ATP-dependent manner.
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Product Notes
The bag-1 bag-1 (Catalog #AAA668524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bag-1 reacts with Cow, Dog, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bag-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200 IF/ICC: 1: 500-1: 2000. Researchers should empirically determine the suitability of the bag-1 bag-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bag-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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