Western Blot (WB)
(Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution. Positive control�� Line 1: Mouse brain Line 2: Jurkat Line 3: Hela)
Rabbit MIF Monoclonal Antibody | anti-MIF antibody
MIF Antibody
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution. Positive control�� Line 1: Mouse brain Line 2: Jurkat Line 3: Hela)
Western Blot (WB)
(Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution. Positive control�� Line 1: Mouse brain Line 2: Jurkat Line 3: Hela)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
Uniprot Description
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.
Protein type: Amino Acid Metabolism - tyrosine; Apoptosis; Cytokine; EC 5.3.3.12; Amino Acid Metabolism - phenylalanine; Cell development/differentiation; Isomerase; EC 5.3.2.1
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle
Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity
Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of MAP kinase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion
Disease: Rheumatoid Arthritis, Systemic Juvenile
Research Articles on MIF
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Product Notes
The MIF mif (Catalog #AAA9436245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MIF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the MIF mif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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