Western Blot (WB)
(Fig: 1 Western blot analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1: 2000.)
Rabbit anti-Human Mcl-1 Polyclonal Antibody | anti-MCL1 antibody
Polyclonal Antibody to Mcl-1
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
IF/ICC: 1: 500-1: 3000
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1: 2000.)
Western Blot (WB)
(Fig: 1 Western blot analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1: 2000.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression using Mcl-1 antibody at 1: 2000. A-D, Mcl-1 staining shown as varying magnifications. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression using Mcl-1 antibody at 1: 2000. A-D, Mcl-1 staining shown as varying magnifications. Hematoxylin-eosin counterstain.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Uniprot Description
MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Protein type: Apoptosis; Channel, misc.; Inhibitor; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: cytoplasm; cytosol; membrane; mitochondrial outer membrane; mitochondrion; nucleus
Molecular Function: BH3 domain binding; protein binding; protein channel activity; protein heterodimerization activity; protein homodimerization activity
Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis; negative regulation of autophagy; response to cytokine stimulus
Research Articles on MCL1
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Product Notes
The MCL1 mcl1 (Catalog #AAA668710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mcl-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mcl-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200 IF/ICC: 1: 500-1: 3000. Researchers should empirically determine the suitability of the MCL1 mcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mcl-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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