Western Blot (WB)
(Western blot analysis of whole cell lysates probed with RIPK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 21 second exposure. Arrows point to RIPK1 (molecular weight 78 and 38 kDa))
Mouse anti-Human, Mouse RIPK1 Monoclonal Antibody | anti-RIPK1 antibody
RIPK1 Antibody with Control Lysate
Western Blotting: Detects a bands of approximately 78 and 38 kDa in HEK293 cell lysates.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with RIPK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 21 second exposure. Arrows point to RIPK1 (molecular weight 78 and 38 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with RIPK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 21 second exposure. Arrows point to RIPK1 (molecular weight 78 and 38 kDa))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
Uniprot Description
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
Research Articles on RIPK1
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Product Notes
The RIPK1 ripk1 (Catalog #AAA225488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RIPK1 Antibody with Control Lysate reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RIPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a bands of approximately 78 and 38 kDa in HEK293 cell lysates. Researchers should empirically determine the suitability of the RIPK1 ripk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIPK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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