Rabbit Atg5 Polyclonal Antibody | anti-Atg5 antibody

Atg5

Cat. Num. AAA191004

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Applications: Immunoblot, Western Blot
• Synonyms: Atg5; Polyclonal Antibody; anti-Atg5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human Atg5.
• Form/Format: Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-Atg5 antibody: Immunoblotting (IB)
• Application Notes: Immunoblotting: use at 1-2ug/ml. A band of ~32kDa, corresponding to free Atg5 is detected. A band of ~60kDa, corresponding to Atg12-Atg5 complex, is also detected.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Synthetic peptide corresponding to mid-protein amino acids of human Atg5.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Testing Data

Related Product Information for anti-Atg5 antibody

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. The protein encoded by the Atg5 gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP

NCBI Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; membrane; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: APG8 conjugating enzyme activity; protein binding

Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation; post-translational protein modification