Rabbit anti-Mouse CD283 Polyclonal Antibody | anti-CD283 antibody

RABBIT ANTI HUMAN CD283

Cat. Num. AAA221327

• Gene Names: TLR3; CD283; IIAE2
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD283; Polyclonal Antibody; RABBIT ANTI HUMAN CD283; anti-CD283 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 627

• Applicable Applications for anti-CD283 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 103.8kDa in human Daudi cell lysates.; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to a 15 amino acid sequence located near human CD283 carboxy-terminus.; Buffer Solution
• Antiserum Preparation: Antisera to human CD283 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Daudi human Burkitt's lymphoma derived cells probed with Rabbit anti Human CD283 (MBS221327) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Immunocytochemical staining of EL4 mouse lymphoblast cells with Rabbit anti Human CD283 (MBS221327))

Related Product Information for anti-CD283 antibody

Rabbit anti Human CD283 antibody recognizes human CD283, otherwise known as toll receptor 3, a 103.8kDa member of the evolutionarily conserved toll-like receptor family (TLRs), expressed at high levels in the placenta and pancreas and also expressed by dendritic cells. TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus. Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 4507531
• NCBI GeneID: 7098
• NCBI Accession #: NP_003256.1
• NCBI GenBank Nucleotide #: NM_003265.2
• UniProt Accession #: O15455; Q4VAL2; Q504W0; B2RAI7; B7Z7K0; E6Y0F0; E6Y0F1; E9PGH4
• Molecular Weight: 72,937 Da
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: TLR3
• NCBI Official Synonym Symbols: CD283; IIAE2
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• UniProt Gene Name: TLR3
• UniProt Entry Name: TLR3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; lysosomal membrane; cytoplasm; endosome membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity

Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; positive regulation of interferon-beta biosynthetic process; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To