Rabbit ATG5 Antibody | anti-ATG5 antibody

ATG5 Antibody

Cat. Num. AAA8505000

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ATG5; ATG5 Antibody; APG5; APG5-LIKE; APG5L; ASP; hAPG5; anti-ATG5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 275

• Applicable Applications for anti-ATG5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ATG5
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATG5 antibody.Immunohistochemistry of paraffin-embedded Colon cancer using ATG5 Antibody.)

Related Product Information for anti-ATG5 antibody

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1, 2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7, 8).

Product Categories/Family for anti-ATG5 antibody

Autophagy antibody

References

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5. Mizushima, N. et al. (1998) Nature 395, 395-8. 6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 32kDa
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP

NCBI Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.