Mouse ACTA2 Antibody | anti-ACTA2 antibody

ACTA2 Antibody

Cat. Num. AAA850889

• Gene Names: ACTA2; ACTSA
• Reactivity: Human, Monkey, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Synonyms: ACTA2; ACTA2 Antibody; AAT6; ACTSA; anti-ACTA2 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Rat, Mouse
• Isotype: IgG1
• Clone Number: 1H8
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 377

• Applicable Applications for anti-ACTA2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human ACTA2 expressed in E. Coli.
• Preparation and Storage: Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot (WB)

(Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.)

Immunofluorescence (IF)

(Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Testing Data

Related Product Information for anti-ACTA2 antibody

Actin, alpha 2, smooth muscle, aorta, major constituent of thin filaments.

Product Categories/Family for anti-ACTA2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 213688375
• NCBI GeneID: 59
• NCBI Accession #: NP_001135417.1
• NCBI GenBank Nucleotide #: NM_001141945.2
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42kDa
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: ACTSA
• NCBI Protein Information: actin, aortic smooth muscle
• UniProt Protein Name: Actin, aortic smooth muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Uniprot Description

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.