Rabbit anti-Human VPS25 Polyclonal Antibody | anti-VPS25 antibody

Anti-VPS25 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108155

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: VPS25; Polyclonal Antibody; Anti-VPS25 Antibody; Rabbit Polyclonal; VPS25 Antibody; Rabbit PAb; Antigen Affinity Purified; vacuolar protein sorting 25 homolog (S. cerevisiae); Anti-DERP9 Antibody; Anti-EAP20 Antibody; Anti-FAP20 Antibody; anti-VPS25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VPS25
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-VPS25 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human VPS25 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human VPS25 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-VPS25 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: THP-1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human VPS25 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(VPS25 was immunoprecipitated using: Lane A:0.5 mg THP-1 Whole Cell Lysate 4 uL anti-VPS25 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS25 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa)

Related Product Information for anti-VPS25 antibody

Vacuolar protein sorting 25 (Vps25), a component of ESCRT-II (Endosomal Sorting Complex Required for Transport II), directs preferential endosome-mediated modulation of FGF signaling in limbs. Vps25p is the product of yeast gene VPS25 and is found in an endosomal sorting complex required for transport (ESCRT)-II, along with Vps22p and Vps36p. This complex is essential for sorting ubiquitinated biosynthetic and endosomal cargoes into endosomes. In Drosophila, mutations of the ESCRT-II component vps25 cause endosomal defects leading to accumulation of Notch protein and increased Notch pathway activity. In Drosophila imaginal epithelia, cells mutant for the endocytic neoplastic tumor suppressor gene vps25 stimulate nearby untransformed cells to express Drosophila Inhibitor-of-Apoptosis-Protein-1 (DIAP-1), conferring resistance to apoptosis non-cell autonomously.

References

Slater R, et al. (2006) Genetic structure and evolution of the vps25 family, a yeast escrt-ii component. BMC Evol Biol 6 59.Herz HM, et al. (2009) Common and distinct genetic properties of escrt-ii components in drosophila. PLoS One 4 (1): e4165.Handschuh K, et al. (2014) Escrt-ii/vps25 constrains digit number by endosome-mediated selective modulation of fgf-shh signaling. Cell Rep 9 (2): 674-687.Graves HK, et al. (2012) Notch signaling activates yorkie non-cell autonomously in drosophila. PLoS One 7 (6): e37615.