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Western Blot (WB) (Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.)

anti-Human, Mouse CAF1A Antibody | anti-CAF1A antibody

CAF1A Antibody

Gene Names
CHAF1A; CAF1; P150; CAF-1; CAF1B; CAF1P150
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CAF1A; CAF1A Antibody; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150; anti-CAF1A antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
CAF1A antibody detects endogenous levels of total CAF1A protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
956
Applicable Applications for anti-CAF1A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human CAF1A.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.)

Western Blot (WB) (Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.)
References
Dong H., Gene 264:187-196(2001) [PubMed: 11250073]. Grimwood J., Nature 428:529-535(2004) [PubMed: 15057824]. Kaufman P.D., Cell 81:1105-1114(1995) [PubMed: 7600578]. Grimwood J., Nature 428:529-535(2004) [PubMed: 15057824]. Kaufman P.D., Cell 81:1105-1114(1995) [PubMed: 7600578].

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,269 Da
NCBI Official Full Name
chromatin assembly factor 1 subunit A
NCBI Official Synonym Full Names
chromatin assembly factor 1 subunit A
NCBI Official Symbol
CHAF1A
NCBI Official Synonym Symbols
CAF1; P150; CAF-1; CAF1B; CAF1P150
NCBI Protein Information
chromatin assembly factor 1 subunit A
UniProt Protein Name
Chromatin assembly factor 1 subunit A
UniProt Gene Name
CHAF1A
UniProt Synonym Gene Names
CAF; CAF1P150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; hp150

NCBI Description

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]

Uniprot Description

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci ().

Research Articles on CAF1A

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Product Notes

The CAF1A chaf1a (Catalog #AAA855643) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CAF1A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAF1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the CAF1A chaf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAF1A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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