Immunofluorescence (IF)
(ASPM / MCPH5 antibody (pAb) tested by immunofluorescence. Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 ug/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Rabbit anti-Human ASPM/MCPH5 Polyclonal Antibody | anti-ASPM/MCPH5 antibody
ASPM/MCPH5 antibody (pAb)
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A Chromatography
Synonyms
ASPM/MCPH5; Polyclonal Antibody; ASPM/MCPH5 antibody (pAb); ASP; MCPH5; Calmbp1; anti-ASPM/MCPH5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-ASPM/MCPH5 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Applications Validated:
ICC/IF: 2ug/ml dilution
ICC/IF: 2ug/ml dilution
Immunogen
This ASPM/MCPH5 antibody was raised against a peptide within the C-terminal region of human ASPM/MCPH5.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunofluorescence (IF)
(ASPM / MCPH5 antibody (pAb) tested by immunofluorescence. Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 ug/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Immunofluorescence (IF)
(ASPM / MCPH5 antibody (pAb) tested by immunofluorescence. Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 ug/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Immunofluorescence (IF)
(ASPM / MCPH5 antibody (pAb) tested by immunofluorescence. Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 ug/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Immunofluorescence (IF)
(ASPM / MCPH5 antibody (pAb) tested by immunofluorescence. Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 ug/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Related Product Information for anti-ASPM/MCPH5 antibody
Short Description: Our ASPM/MCPH5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry and Immunofluorescence; it has been shown to react with Human samples.
Background: ASPM (abnormal spindle-like microcephaly associated, microcephaly primary type 5, MCPH5) is a protein that participates in spindle organization, spindle positioning and cytokinesis in all dividing cells. ASPM/MCPH5 localizes to centrosomes, spindle poles, and the spindle midbody. It regulates the orientation of the mitotic spindle, which controls whether cells divide symmetrically or asymmetrically. ASPM/MCPH5 is highly expressed in many tumor cell lines and mutations in the gene encoding ASPM/MCPH5 lead to primary microcephaly in humans.
Background: ASPM (abnormal spindle-like microcephaly associated, microcephaly primary type 5, MCPH5) is a protein that participates in spindle organization, spindle positioning and cytokinesis in all dividing cells. ASPM/MCPH5 localizes to centrosomes, spindle poles, and the spindle midbody. It regulates the orientation of the mitotic spindle, which controls whether cells divide symmetrically or asymmetrically. ASPM/MCPH5 is highly expressed in many tumor cell lines and mutations in the gene encoding ASPM/MCPH5 lead to primary microcephaly in humans.
Product Categories/Family for anti-ASPM/MCPH5 antibody
NCBI and Uniprot Product Information
Molecular Weight
460kDa
Similar Products
Product Notes
The ASPM/MCPH5 (Catalog #AAA388808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASPM/MCPH5 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASPM/MCPH5 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). Applications Validated: ICC/IF: 2ug/ml dilution. Researchers should empirically determine the suitability of the ASPM/MCPH5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASPM/MCPH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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