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Immunofluorescence (IF) (ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence. HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain Immunofluorescence Tools. Panel A: Normal HeLa cells. Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure. Top images: Cells stained with DAPI. Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12). Bottom images: Merge of the two images above. Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.)

Mouse anti-Human, Mouse ATM phospho Ser1981 Monoclonal Antibody | anti-ATM antibody

ATM phospho Ser1981 antibody (mAb)(Clone 10H11.E.12)

Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Ascites
Synonyms
ATM phospho Ser1981; Monoclonal Antibody; ATM phospho Ser1981 antibody (mAb)(Clone 10H11.E.12); Ataxia Telangiectasia Mutated; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; anti-ATM antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
10H11.E.12
Purity/Purification
Ascites
Form/Format
Ascites containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-ATM antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 1:500 dilution
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Conjugation
Phosphorylated
Immunogen
This ATM phospho Ser1981 antibody was raised against a peptide containing phospho-serine 1981 of human ATM.
Abs Residue
Ser
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice

Immunofluorescence (IF)

(ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence. HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain Immunofluorescence Tools. Panel A: Normal HeLa cells. Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure. Top images: Cells stained with DAPI. Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12). Bottom images: Merge of the two images above. Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.)

Immunofluorescence (IF) (ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence. HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain Immunofluorescence Tools. Panel A: Normal HeLa cells. Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure. Top images: Cells stained with DAPI. Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12). Bottom images: Merge of the two images above. Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.)
Related Product Information for anti-ATM antibody
Short Description: Our ATM phospho Ser1981 antibody (mAb)(Clone 10H11.E.12) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.

Background: ATM-Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1 and SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.
Product Categories/Family for anti-ATM antibody

NCBI and Uniprot Product Information

Molecular Weight
370kDa
Protein Family

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Product Notes

The ATM (Catalog #AAA388478) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATM phospho Ser1981 antibody (mAb)(Clone 10H11.E.12) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATM phospho Ser1981 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 1:500 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the ATM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM phospho Ser1981, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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