Immunofluorescence (IF)
(CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence. Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 ug/ml dilution (red) and DAPI (blue).)
Mouse anti-Human, Mouse CENP-E Monoclonal Antibody | anti-CENP-E antibody
CENP-E antibody (mAb)(Clone 1H12)
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein G Chromatography
Synonyms
CENP-E; Monoclonal Antibody; CENP-E antibody (mAb)(Clone 1H12); KIF10; CENPE; PPP1R61; anti-CENP-E antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
1H12
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-CENP-E antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Applications Validated:
ICC/IF: 1ug/ml dilution
This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.
ICC/IF: 1ug/ml dilution
This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.
Immunogen
This CENP-E antibody was raised against full-length human CENP-E protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunofluorescence (IF)
(CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence. Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 ug/ml dilution (red) and DAPI (blue).)
Immunofluorescence (IF)
(CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence. Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 ug/ml dilution (red) and DAPI (blue).)
Immunofluorescence (IF)
(Detection of CENP-E by immunofluorescence. U2OS cells were stained with CENP-E antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: CENP-E antibody staining. Right panel: merge.)
Immunofluorescence (IF)
(Detection of CENP-E by immunofluorescence. U2OS cells were stained with CENP-E antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: CENP-E antibody staining. Right panel: merge.)
Related Product Information for anti-CENP-E antibody
Short Description: Our CENP-E antibody (mAb)(Clone 1H12) was raised in a Mouse host. It has been validated for use in Immunocytochemistry and Immunofluorescence; it has been shown to react with Human and Mouse samples.
Background: Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.
Background: Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.
Product Categories/Family for anti-CENP-E antibody
NCBI and Uniprot Product Information
Molecular Weight
320kDa
Similar Products
Product Notes
The CENP-E (Catalog #AAA388513) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CENP-E antibody (mAb)(Clone 1H12) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CENP-E can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). Applications Validated: ICC/IF: 1ug/ml dilution This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation. Researchers should empirically determine the suitability of the CENP-E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENP-E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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