Immunofluorescence (IF)
(Nucleolin mAb (Clone 3G4B20) tested by immunofluorescence. Staining of HeLa cells with Nucleolin mAb (Clone 3G4B20) (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Mouse Nucleolin Monoclonal Antibody | anti-Nucleolin antibody
Nucleolin antibody (mAb)(Clone 3G4B20)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Culture Supernatant
Synonyms
Nucleolin; Monoclonal Antibody; Nucleolin antibody (mAb)(Clone 3G4B20); NCL; C23; anti-Nucleolin antibody
Host
Mouse
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G4B20
Purity/Purification
Culture Supernatant
Form/Format
Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Nucleolin antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 1:500 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ICC/IF: 1:500 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Immunofluorescence (IF)
(Nucleolin mAb (Clone 3G4B20) tested by immunofluorescence. Staining of HeLa cells with Nucleolin mAb (Clone 3G4B20) (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Immunofluorescence (IF)
(Nucleolin mAb (Clone 3G4B20) tested by immunofluorescence. Staining of HeLa cells with Nucleolin mAb (Clone 3G4B20) (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Western Blot (WB)
(Nucleolin mAb (Clone 3G4B20) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with Nucleolin mAb (Clone 3G4B20) (1:2,000 dilution).)
Western Blot (WB)
(Nucleolin mAb (Clone 3G4B20) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with Nucleolin mAb (Clone 3G4B20) (1:2,000 dilution).)
Related Product Information for anti-Nucleolin antibody
Short Description: Our Nucleolin antibody (mAb)(Clone 3G4B20) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.
Background: Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.
Product Categories/Family for anti-Nucleolin antibody
NCBI and Uniprot Product Information
Molecular Weight
100kDa
Protein Family
Similar Products
Product Notes
The Nucleolin (Catalog #AAA388483) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nucleolin antibody (mAb)(Clone 3G4B20) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleolin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 1:500 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the Nucleolin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleolin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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