Immunofluorescence (IF)
(Cyk-4 phospho Ser170 pAb tested by immunofluorescence. Normal human retinal pigment epithelial cells stained with 39265 (1:300). Left: 39265. Right: merge with DAPI.)
Rabbit anti-Human Cyk-4 phospho Ser170 Polyclonal Antibody | anti-Cyk-4 antibody
Cyk-4 phospho Ser170 antibody (pAb)
ICC/IF: 1:200-1:500 dilution
WB: 1:1,000-1:2,500 dilution
DB: 1:2000
This Cyk-4 antibody must first be immunoprecipitated and then run on a gel before Western blotting.
Immunofluorescence (IF)
(Cyk-4 phospho Ser170 pAb tested by immunofluorescence. Normal human retinal pigment epithelial cells stained with 39265 (1:300). Left: 39265. Right: merge with DAPI.)
Immunofluorescence (IF)
(Cyk-4 phospho Ser170 pAb tested by immunofluorescence. Normal human retinal pigment epithelial cells stained with 39265 (1:300). Left: 39265. Right: merge with DAPI.)
Western Blot (WB)
(Cyk-4 phospho Ser170 Rabbit pAb tested by Western Blot. Recombinant Cyk-4 fragment (100 ng) untreated (lane 1) and incubated with the kinase Plk-1 (lane 2) was probed with 39265 at 1:2,000.)
Western Blot (WB)
(Cyk-4 phospho Ser170 Rabbit pAb tested by Western Blot. Recombinant Cyk-4 fragment (100 ng) untreated (lane 1) and incubated with the kinase Plk-1 (lane 2) was probed with 39265 at 1:2,000.)
Dot Blot (DB)
(Cyk-4 phospho Ser170 pAb tested by dot blot to show specificity for serine 170. Dot blot analysis was used to confirm the specificity of 39265 for phospho Ser170 Cyk-4. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with 39265 at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is 250,50,10 and 2 from top to bottom. Lane 1: phospho Ser170 peptide. Lane 2: unmodified Ser170 peptide.)
Dot Blot (DB)
(Cyk-4 phospho Ser170 pAb tested by dot blot to show specificity for serine 170. Dot blot analysis was used to confirm the specificity of 39265 for phospho Ser170 Cyk-4. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with 39265 at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is 250,50,10 and 2 from top to bottom. Lane 1: phospho Ser170 peptide. Lane 2: unmodified Ser170 peptide.)
Background: Cyk-4 is a member of a GTPase activating protein family that regulates the catalytic function of Rho and Ras small G-proteins. Cyk-4 regulates the initiation of cytokinesis through its interaction with a variety of microtubule organizing proteins. The activity of Cyk-4 is regulated by phophorylation of serine 170 by Polo-like Kinase 1.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
Uniprot Description
MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.
Protein type: GAPs; GAPs, Rac/Rho
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; spindle midzone; midbody; cytosol; nucleus; cleavage furrow
Molecular Function: gamma-tubulin binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; microtubule binding; beta-tubulin binding; protein kinase binding; alpha-tubulin binding; GTPase activator activity
Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; sulfate transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule-based movement; regulation of small GTPase mediated signal transduction; embryonic development; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; cytokinesis, contractile ring formation; positive regulation of GTPase activity
Research Articles on Cyk-4
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Product Notes
The Cyk-4 racgap1 (Catalog #AAA388436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyk-4 phospho Ser170 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyk-4 phospho Ser170 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 1:200-1:500 dilution WB: 1:1,000-1:2,500 dilution DB: 1:2000 This Cyk-4 antibody must first be immunoprecipitated and then run on a gel before Western blotting. Researchers should empirically determine the suitability of the Cyk-4 racgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyk-4 phospho Ser170, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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