Rabbit anti-Human ARHB Polyclonal Antibody | anti-ARHB antibody

ARHB, ID (RHOB, ARH6, ARHB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6) (MaxLight 650)

Cat. Num. AAA6274827

• Gene Names: RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ARHB; Polyclonal Antibody; ID (RHOB; ARH6; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6) (MaxLight 650); RHOB; Rho cDNA clone 6; anti-ARHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ARHB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ARHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-137 amino acids from the Central region of human ARHB.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ARHB Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARHB antibody detected the ARHB protein (arrow).)

Immunohistochemistry (IHC)

(ARHB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ARHB antibody

ARHB mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.

Product Categories/Family for anti-ARHB antibody

Antibodies; CD Markers

References

Adly, M.A., et al. J. Cutan. Pathol. 37(7):751-757(2010). Connolly, E.C., et al. Mol. Cancer Res. 8(5):691-700(2010). Zintzaras, E., et al. Am. J. Epidemiol. 171(8):851-858(2010). Kim, C.H., et al. Biochem. Biophys. Res. Commun. 391(2):1182-1186(2010). Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4757764
• NCBI GeneID: 388
• NCBI Accession #: NP_004031.1
• NCBI GenBank Nucleotide #: NM_004040.2
• UniProt Accession #: P62745; P01121; Q5U0H6; Q7RTN5; Q7RTR9; Q9CUV7; B2R692
• Molecular Weight: 22,123 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoB
• NCBI Official Synonym Full Names: ras homolog family member B
• NCBI Official Symbol: RHOB
• NCBI Official Synonym Symbols: ARH6; ARHB; RHOH6; MST081; MSTP081
• NCBI Protein Information: rho-related GTP-binding protein RhoB; h6; oncogene RHO H6; rho cDNA clone 6; Aplysia RAS-related homolog 6; ras homolog gene family, member B
• UniProt Protein Name: Rho-related GTP-binding protein RhoB
• UniProt Gene Name: RHOB
• UniProt Synonym Gene Names: ARH6; ARHB; h6
• UniProt Entry Name: RHOB_HUMAN

Uniprot Description

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: Tumor suppressor; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; nucleus; cytosol; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: axon guidance; platelet activation; transformed cell apoptosis; metabolic process; apoptosis; positive regulation of apoptosis; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; positive regulation of angiogenesis; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; blood coagulation; cell adhesion