Rabbit CUEDC2 Polyclonal Antibody | anti-CUEDC2 antibody

CUEDC2 Antibody

Cat. Num. AAA150743

• Gene Names: CUEDC2; C10orf66; bA18I14.5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: CUEDC2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CUEDC2; Polyclonal Antibody; CUEDC2 Antibody; C10orf66; bA18I14.5; HOYS6; CUE domain-containing protein 2; CUE domain containing 2; anti-CUEDC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CUEDC2 antibody will not cross-react with CUEDC1.
• Purity/Purification: CUEDC2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 287

• Applicable Applications for anti-CUEDC2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CUEDC2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2.
• Buffer: CUEDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CUEDC2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.)

Related Product Information for anti-CUEDC2 antibody

CUEDC2 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-κB by decreasing the activation of IKK.

NCBI and Uniprot Product Information

• NCBI GI #: 148596996
• NCBI GeneID: 79004
• NCBI Accession #: NP_076945
• NCBI GenBank Nucleotide #: NM_024040.2
• UniProt Accession #: Q9H467; Q9BWG8; D3DR88
• Molecular Weight: 32,009 Da
• NCBI Official Full Name: CUE domain-containing protein 2
• NCBI Official Synonym Full Names: CUE domain containing 2
• NCBI Official Symbol: CUEDC2
• NCBI Official Synonym Symbols: C10orf66; bA18I14.5
• NCBI Protein Information: CUE domain-containing protein 2
• UniProt Protein Name: CUE domain-containing protein 2
• Protein Family: CUE domain-containing protein
• UniProt Gene Name: CUEDC2
• UniProt Synonym Gene Names: C10orf66
• UniProt Entry Name: CUED2_HUMAN

Uniprot Description

CUEDC2: Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. May predict the clinical outcome of tamoxifen therapy of breast cancer patients. Patients with tumors that highly express CUEDC2 do not respond to tamoxifen treatment as effectively as those with tumors with low expression. Belongs to the CUEDC2 family.

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm

Molecular Function: protein binding