Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit | ITaC elisa kit

Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

Cat. Num. AAA2021878

• Gene Names: CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• Reactivity: Human
• Synonyms: Interferon Inducible T-Cell Alpha Chemoattractant (ITaC); Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit; CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9; ITaC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC).; No significant cross-reactivity or interference between Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) and analogues was observed.
• Sequence Length: 94

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 26.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITaC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible T-Cell Alpha Chemoattractant (ITaC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ITaC elisa kit

Cytokine; Signal transduction; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 3219693
• NCBI GeneID: 6373
• UniProt Accession #: O14625; Q53YA3; Q92840
• Molecular Weight: 10,365 Da
• NCBI Official Full Name: interferon stimulated T-cell alpha chemoattractant
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 11
• NCBI Official Symbol: CXCL11
• NCBI Official Synonym Symbols: IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• NCBI Protein Information: C-X-C motif chemokine 11
• UniProt Protein Name: C-X-C motif chemokine 11
• UniProt Gene Name: CXCL11
• UniProt Synonym Gene Names: ITAC; SCYB11; SCYB9B; IP-9; I-TAC
• UniProt Entry Name: CXL11_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; inflammatory response; signal transduction; chemotaxis; regulation of cell proliferation