Rabbit anti-Rat Aquaporin 1 Polyclonal Antibody | anti-AQP1 antibody
Aquaporin 1 (AQP1, CHIP28) (Biotin) (Azide, BSA & Glycerol free)
Gene Names
Aqp1; CHIP28
Reactivity
Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography
Synonyms
Aquaporin 1; Polyclonal Antibody; Aquaporin 1 (AQP1; CHIP28) (Biotin) (Azide; BSA & Glycerol free); anti-AQP1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Aquaporin 1 at ~28.8kD. Species Sequence Homology: human, mouse, bovine, sheep: 100%
Purity/Purification
Purified by Immunoaffinity chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free. Labeled with Biotin.
Applicable Applications for anti-AQP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: Chemiluminescence technique reveals two bands, a lower 28kD band corresponding to unglycosylated form and an upper high molecular 35-60kD glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunogen
Synthetic peptide corresponding to 19aa from the cytosolic carboxyl terminal domain of the AQP1, conjugated to KLH. This peptide contains most of the epitopes recognized by polyclonal antibodies against the whole protein.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot: A3000 (0.5ug/ml) in adult rat heart lysate.)
Related Product Information for anti-AQP1 antibody
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Aquaporin, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of the aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin 1 (APQ1) is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues.
Product Categories/Family for anti-AQP1 antibody
References
1. Mobasheri, A., et al., Vet. J. 168: 143-150 (2004). 2. Mobasheri, A., et al., BJU International 92: 793-802 (2003). 3. Gallardo, P., et al., Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R779-R788 (2002). 4. Zhou, Q., et al., PNAS 98(24): 14, 132-14, 137 (2001). 5. Belyantseva, I.A., et al., J. Neurosci. 20(24): 8996-9003 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,857 Da
NCBI Official Full Name
aquaporin-1
NCBI Official Synonym Full Names
aquaporin 1
NCBI Official Symbol
Aqp1
NCBI Official Synonym Symbols
CHIP28
NCBI Protein Information
aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule
UniProt Protein Name
Aquaporin-1
UniProt Gene Name
Aqp1
UniProt Synonym Gene Names
Chip28; AQP-1
UniProt Entry Name
AQP1_RAT
