Typical Testing Data/Standard Curve (for reference only)
Human Aquaporin 1 ELISA Kit | AQP 1 elisa kit
Human Aquaporin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AQP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AQP1 antibody and an AQP1-HRP conjugate. The assay sample and buffer are incubated together with AQP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP1 concentration since AQP1 from samples and AQP1-HRP conjugate compete for the anti-AQP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP1 from the sample, fewer sites are left to bind AQP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
integral membrane protein that is a major water transport molecule in the kidney proximal tubule and red blood cells [RGD, Feb 2006]
Uniprot Description
AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Transporter; Membrane protein, integral
Cellular Component: neuron projection; nuclear membrane; basolateral plasma membrane; integral to plasma membrane; brush border membrane; cell; integral to membrane; membrane; apical part of cell; axon; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border
Molecular Function: protein binding; potassium channel activity; ephrin receptor binding; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; transmembrane transporter activity; water channel activity; nitric oxide transporter activity; glycerol channel activity
Biological Process: glycerol transport; cGMP biosynthetic process; cerebrospinal fluid secretion; wound healing; water transport; carbon dioxide transport; response to hormone stimulus; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; water homeostasis; odontogenesis; positive regulation of fibroblast proliferation; nitric oxide transport; hyperosmotic salinity response; multicellular organismal water homeostasis; cellular homeostasis; lateral ventricle development; potassium ion transport; lipid digestion; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; hyperosmotic response; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; negative regulation of apoptosis; positive regulation of cell migration
Research Articles on AQP 1
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Product Notes
The Human AQP 1 aqp1 (Catalog #AAA720780) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720780 ELISA Kit recognizes Human AQP 1. It is sometimes possible for the material contained within the vial of "Aquaporin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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