Rabbit anti-Human SSX4 Polyclonal Antibody | anti-SSX4 antibody

SSX4, ID (SSX4, SSX4A, Protein SSX4, Cancer/testis antigen 5.4) (Biotin)

Cat. Num. AAA6351435

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SSX4; Polyclonal Antibody; ID (SSX4; SSX4A; Protein SSX4; Cancer/testis antigen 5.4) (Biotin); Cancer/testis antigen 5.4; anti-SSX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 153

• Applicable Applications for anti-SSX4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SSX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human SSX4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SSX4 Antibody (Center) western blot analysis in CEM,K562,NCI-H292,MDA-MB453,A549 cell line lysates (35ug/lane).This demonstrates the SSX4 antibody detected the SSX4 protein (arrow).)

Related Product Information for anti-SSX4 antibody

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4B, represents the more centromeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-SSX4 antibody

Antibodies; Cancer Markers

References

Ayyoub, M., et al. J. Immunol. 174(8):5092-5099(2005). Ross, M.T., et al. Nature 434(7031):325-337(2005). Gure, A.O., et al. Int. J. Cancer 101(5):448-453(2002). Brodin, B., et al. Gene 268 (1-2), 173-182 (2001): Chen, C.H., et al. Cancer Lett. 164(2):189-195(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 28559013
• NCBI GeneID: 548313; 6759
• NCBI Accession #: NP_783856.1; NP_005627.1
• NCBI GenBank Nucleotide #: NP_783856.1
• UniProt Accession #: O60224
• NCBI Official Full Name: protein SSX4 isoform b
• UniProt Protein Name: Protein SSX4
• Protein Family: Protein
• UniProt Gene Name: SSX4
• UniProt Synonym Gene Names: SSX4A; CT5.4

Uniprot Description

SSX4: Could act as a modulator of transcription. Belongs to the SSX family.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleus

Molecular Function: nucleic acid binding; transcription corepressor activity

Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent