NAD(P)H-flavin reductase (fre) Recombinant Protein | fre recombinant protein

Recombinant Escherichia coli NAD(P)H-flavin reductase (fre)

Cat. Num. AAA1265545

• Gene Names: fre; ECK3836; fadI; flrD; fsrC; hsm-6; JW3820
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: NAD(P)H-flavin reductase (fre); Recombinant Escherichia coli NAD(P)H-flavin reductase (fre); Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase CNAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H]; fre recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 2-233. Full Length of Mature Protein
• Sequence: TTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDERDKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDEERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHPGLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSERNAREDRLFGDAFAFI

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for fre recombinant protein

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Ses to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.

References

Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.Spyrou G., Haggaard-Ljungquist E., Krook M., Joernvall H., Nilsson E., Reichard P.J. Bacteriol. 173:3673-3679(1991) FadI identification, characterization, and nucleotide sequence of a gene required for full activation of structural genes of the AD regulon in Escherichia coli.Dirusso C.C., Shea O. Saviranta P.J. Analysis of the Escherichia coli genome DNA sequence of the region from 84.5 to 86.5 minutes.Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.Science 257:771-778(1992) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases.Andrews S.C., Shipley D., Keen J.N., Findlay J.B.C., Harrison P.M., Guest J.R.FEBS Lett. 302:247-252(1992) Crystal structure of NAD(P) H:flavin oxidoreductase from Escherichia coli.Ingelman M., Ramaswamy S., Niviere V., Fontecave M., Eklund H.Biochemistry 38:7040-7049(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 16131690
• NCBI GeneID: 948325
• NCBI Accession #: NP_418286.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AEN1; P23486; P76768; Q2M8E7
• Molecular Weight: 30.1 kDa
• NCBI Official Full Name: NAD(P)H-flavin reductase
• NCBI Official Symbol: fre
• NCBI Official Synonym Symbols: ECK3836; fadI; flrD; fsrC; hsm-6; JW3820
• NCBI Protein Information: NAD(P)H-flavin reductase
• UniProt Protein Name: NAD(P)H-flavin reductase
• Protein Family: NAD(P)H-flavin reductase
• UniProt Gene Name: fre
• UniProt Synonym Gene Names: fadI; flrD; fsrC; ubiB
• UniProt Entry Name: FRE_ECOLI

NCBI Description

The fre gene was mistakenly thought to be ubiB. [More information is available at EcoGene: EG10334].

Uniprot Description

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.