Rabbit Aquaporin 1 Polyclonal Antibody | anti-Aqp1 antibody

Aquaporin 1 Antibody: Alkaline Phosphatase

Cat. Num. AAA805974

• Gene Names: Aqp1; CHIP28
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Synonyms: Aquaporin 1; Polyclonal Antibody; Aquaporin 1 Antibody: Alkaline Phosphatase; AQP1; AQP 1; AQP CHIP; AQP-1; AQP1_HUMAN; Aquaporin CHIP; Aquaporin-1; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule; anti-Aqp1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 269

• Applicable Applications for anti-Aqp1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB 1:1000-4000, IF: 1:200-400
• Conjugate: Alkaline Phosphatase
• Preparation and Storage: 4 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)

Western Blot (WB)

(Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:2000. Showing glycosylated and non-glycosylated bands.)

Related Product Information for anti-Aqp1 antibody

Background Info: Detects ~28.5kDa. May detect larger glycosylated bands ~35-50kDa.

Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).

Product Categories/Family for anti-Aqp1 antibody

Ion Channels and Transporters

References

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. www.vivo.colostate.edu/hbooks/molecules/aqu aporins.html

NCBI and Uniprot Product Information

• NCBI GI #: 6978527
• NCBI GeneID: 25240
• NCBI Accession #: NP_036910.1
• NCBI GenBank Nucleotide #: NM_012778.1
• UniProt Accession #: P29975; Q5EB50; Q7TN36
• Molecular Weight: 28,857 Da
• NCBI Official Full Name: aquaporin-1
• NCBI Official Synonym Full Names: aquaporin 1
• NCBI Official Symbol: Aqp1
• NCBI Official Synonym Symbols: CHIP28
• NCBI Protein Information: aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: Aqp1
• UniProt Synonym Gene Names: Chip28; AQP-1
• UniProt Entry Name: AQP1_RAT

NCBI Description

integral membrane protein that is a major water transport molecule in the kidney proximal tubule and red blood cells [RGD, Feb 2006]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Cellular Component: nuclear membrane; neuron projection; cell; brush border membrane; basolateral plasma membrane; integral to plasma membrane; integral to membrane; membrane; apical part of cell; axon; cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; potassium channel activity; ephrin receptor binding; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; transmembrane transporter activity; nitric oxide transporter activity; water channel activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; carbon dioxide transport; water transport; response to hormone stimulus; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; odontogenesis; water homeostasis; positive regulation of fibroblast proliferation; nitric oxide transport; hyperosmotic salinity response; multicellular organismal water homeostasis; cellular homeostasis; potassium ion transport; lipid digestion; lateral ventricle development; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; hyperosmotic response; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; response to estrogen stimulus; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; negative regulation of apoptosis; positive regulation of cell migration