Rabbit anti-Human CDC27 Polyclonal Antibody | anti-CDC27 antibody

CDC27, CT (Cell Division Cycle Protein 27 Homolog, Anaphase-promoting Complex Subunit 3, APC3, CDC27 Homolog, CDC27Hs, H-NUC, ANAPC3, D0S1430E, D17S978E)

Cat. Num. AAA6007038

• Gene Names: CDC27; APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CDC27; Polyclonal Antibody; CT (Cell Division Cycle Protein 27 Homolog; Anaphase-promoting Complex Subunit 3; APC3; CDC27 Homolog; CDC27Hs; H-NUC; ANAPC3; D0S1430E; D17S978E); Anti -CDC27; anti-CDC27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CDC27. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CDC27 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 19aa from human APC3 at C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2ug/ml.)

Related Product Information for anti-CDC27 antibody

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Product Categories/Family for anti-CDC27 antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 405833
• NCBI GeneID: 996
• UniProt Accession #: P30260; Q16349; Q96F35; G3V1C4
• Molecular Weight: 91,867 Da
• NCBI Official Full Name: CDC27
• NCBI Official Synonym Full Names: cell division cycle 27
• NCBI Official Symbol: CDC27
• NCBI Official Synonym Symbols: APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• NCBI Protein Information: cell division cycle protein 27 homolog; H-NUC; nuc2 homolog; CDC27 homolog; cell division cycle 27 homolog; anaphase promoting complex subunit 3; anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3
• UniProt Protein Name: Cell division cycle protein 27 homolog
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC27
• UniProt Synonym Gene Names: ANAPC3; D0S1430E; D17S978E; APC3; CDC27Hs
• UniProt Entry Name: CDC27_HUMAN

NCBI Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC27: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI. Belongs to the APC3/CDC27 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; mitotic metaphase/anaphase transition