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Immunohistochemistry (IHC) (Anti-ACOT9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Mouse anti-Human ACOT9 Monoclonal Antibody | anti-ACOT9 antibody

Mouse Monoclonal [clone 4E4] (IgG1,k) to Human ACOT9

Gene Names
ACOT9; ACATE2; CGI-16; MTACT48; MT-ACT48
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
ACOT9; Monoclonal Antibody; Mouse Monoclonal [clone 4E4] (IgG1; k) to Human ACOT9; Anti-ACOT9 Antibody (clone 4E4) IHC-plus; ACATE2; Acyl-CoA thioester hydrolase 9; Acyl-CoA thioesterase 9; MTACT48; CGI-16; MT-ACT48; Human ACOT9; anti-ACOT9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4E4
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
439
Applicable Applications for anti-ACOT9 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Target Species
Human
Immunogen Description
ACATE2 (AAH12573, 1 a.a. ~ 213 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
ACOT9 antibody was raised against aCATE2 (AAH12573, 1 a.a. ~ 213 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-ACOT9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-ACOT9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ACOT9 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-ACOT9 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell (antibody concentration 10 ug/ml).)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody, clone 4E4.)

Western Blot (WB) (Western blot of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody, clone 4E4.)

Western Blot (WB)

(ACATE2 monoclonal antibody, clone 4E4 Western blot of ACATE2 expression in MCF-7.)

Western Blot (WB) (ACATE2 monoclonal antibody, clone 4E4 Western blot of ACATE2 expression in MCF-7.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ACOT9 is approximately 3 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged ACOT9 is approximately 3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,851 Da
NCBI Official Full Name
acyl-coenzyme A thioesterase 9, mitochondrial isoform b
NCBI Official Synonym Full Names
acyl-CoA thioesterase 9
NCBI Official Symbol
ACOT9
NCBI Official Synonym Symbols
ACATE2; CGI-16; MTACT48; MT-ACT48
NCBI Protein Information
acyl-coenzyme A thioesterase 9, mitochondrial; acyl-CoA thioester hydrolase 9; mitochondrial Acyl-CoA Thioesterase; acyl-Coenzyme A thioesterase 2, mitochondrial
UniProt Protein Name
Acyl-coenzyme A thioesterase 9, mitochondrial
UniProt Gene Name
ACOT9
UniProt Synonym Gene Names
Acyl-CoA thioesterase 9
UniProt Entry Name
ACOT9_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

ACOT9: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs. Belongs to the acyl coenzyme A hydrolase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.2.-

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: mitochondrion

Molecular Function: acetyl-CoA hydrolase activity

Biological Process: acyl-CoA metabolic process

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Product Notes

The ACOT9 acot9 (Catalog #AAA246421) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4E4] (IgG1,k) to Human ACOT9 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACOT9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB Usage: Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). Researchers should empirically determine the suitability of the ACOT9 acot9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACOT9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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