Testing Data
Mouse beta-Tubulin Monoclonal Antibody
beta-Tubulin
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Protein A Purified
Synonyms
beta-Tubulin; Monoclonal Antibody; Tubulin beta; anti-beta-Tubulin antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody detects a 50 kDa* protein corresponding to the molecular mass of b-Tubulin on SDS-PAGE immunoblots of purified brain tubulin, mouse brain tissue, rat PC12 cells, and human A431 and SH-SY5Y cells.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-beta-Tubulin antibody
ELISA, Western Blot, Immunocytochemistry
Application Notes
ELISA: 1:2000
Immunocytochemistry: 1:200
Western Blot: 1:1000
Immunocytochemistry: 1:200
Western Blot: 1:1000
Immunogen
Clone (M154) was generated from purified porcine brain tubulin.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Testing Data 2
Testing Data 2
Related Product Information for anti-beta-Tubulin antibody
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/b-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in b-tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of bIII-tubulin in vitro. Unphosphorylated Ser-444 in bIII-tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in b-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.
References
• Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
• Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
• Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
• Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
• Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
• Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
• Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
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Product Notes
The beta-Tubulin (Catalog #AAA470004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Tubulin reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunocytochemistry. ELISA: 1:2000 Immunocytochemistry: 1:200 Western Blot: 1:1000. Researchers should empirically determine the suitability of the beta-Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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