Testing Data
beta-III-Tubulin (Ser-444) Peptide
Unphosphorylated beta-III-Tubulin (Ser-444) Peptide
Synonyms
beta-III-Tubulin (Ser-444) Peptide; Unphosphorylated beta-III-Tubulin (Ser-444) Peptide; Unphosphorylated bIII-Tubulin (Ser-444) Peptide; beta-III-Tubulin (Ser-444) Peptide peptide
Specificity
The peptide is specifically recognized by unphosphorylated beta III-Tubulin (Ser-444) antibody (TP1811) in ELISA, and has been shown to block the reactivity of TP1811 in Western blot and immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Unphosphorylated beta-III-Tubulin (Ser-444) synthetic peptide contains amino acid residues around serine 444 of human beta-III-tubulin. This sequence is not found in beta-I or beta-II-tubulin isotypes, but is well conserved in beta-III-tubulins from rat and mouse.
Application Notes
Blocking: 1:1,000
ELISA: 50 ng/well
ELISA: 50 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for beta-III-Tubulin (Ser-444) Peptide peptide
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of alpha/beta-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the bestknown factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in beta-tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of beta-III-tubulin in vitro. Unphosphorylated Ser-444 in beta-III-tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in beta-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.
References
• Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041
• Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
• Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
• Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
• Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
• Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
• Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Similar Products
Product Notes
The beta-III-Tubulin (Ser-444) Peptide (Catalog #AAA474003) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Blocking: 1:1,000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the beta-III-Tubulin (Ser-444) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Unphosphor ylated beta-III-T ubulin (Ser-444) synthetic peptide contains amino acid residues around serine 444 of human beta-III-t ubulin. This sequence is not found in beta-I or beta-II-tu bulin isotypes, but is well conserved in beta-III-t ubulins from rat and mouse. It is sometimes possible for the material contained within the vial of "beta-III-Tubulin (Ser-444) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
