Human Alpha-synuclein ELISA Kit | SNCA elisa kit

Human Alpha-synuclein ELISA Kit

Cat. Num. AAA760032

• Reactivity: Human
• Synonyms: Alpha-synuclein; Human Alpha-synuclein ELISA Kit; SNCA/NACP/PARK1/PARK4/PD1/SNCA/Lewy body) 4/non A-beta component of AD amyloid/Non-A beta component of AD amyloid/non-A4 component of amyloid/Non-A4 component of amyloid precursor/PARK1/PARK4/synuclein; alpha (non A4 component of amyloid precursor)/Synuclein-alpha; SNCA elisa kit

• Reactivity: Human
• Sequence Length: 140

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SNCA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SNCA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SNCA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SNCA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SNCA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9507125
• NCBI GeneID: 29219
• NCBI Accession #: NP_062042.1
• NCBI GenBank Nucleotide #: NM_019169.2
• Molecular Weight: 4,354 Da
• NCBI Official Full Name: alpha-synuclein
• NCBI Official Synonym Full Names: synuclein alpha
• NCBI Official Symbol: Snca
• NCBI Protein Information: alpha-synuclein
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: Snca
• UniProt Entry Name: SYUA_RAT

NCBI Description

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Cellular Component: actin cytoskeleton; axon; cell cortex; cell junction; cytoplasm; cytoplasmic vesicle membrane; cytoskeleton; cytosol; extracellular space; fibril; Golgi apparatus; growth cone; inclusion body; intracellular membrane-bound organelle; membrane; mitochondrion; nerve terminal; nuclear outer membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; platelet alpha granule membrane; ribosome; rough endoplasmic reticulum; synapse; synaptic vesicle; terminal button

Molecular Function: alpha-tubulin binding; arachidonic acid binding; beta-tubulin binding; calcium ion binding; caspase inhibitor activity; copper ion binding; dynein binding; enzyme binding; fatty acid binding; ferrous iron binding; histone binding; identical protein binding; kinesin binding; magnesium ion binding; microtubule binding; oxidoreductase activity; phospholipase binding; phospholipid binding; phosphoprotein binding; protein binding; protein domain specific binding; protein N-terminus binding; tau protein binding; zinc ion binding

Biological Process: adult locomotory behavior; aging; behavioral response to cocaine; calcium ion homeostasis; caspase activation; dopamine biosynthetic process; dopamine metabolic process; fatty acid metabolic process; microglial cell activation; mitochondrial membrane organization and biogenesis; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of dopamine metabolic process; negative regulation of dopamine uptake; negative regulation of exocytosis; negative regulation of histone acetylation; negative regulation of microtubule polymerization; negative regulation of monooxygenase activity; negative regulation of neuron apoptosis; negative regulation of norepinephrine uptake; negative regulation of protein amino acid phosphorylation; negative regulation of serotonin uptake; negative regulation of transporter activity; neutral lipid metabolic process; organelle ATP synthesis coupled electron transport; phospholipid metabolic process; positive regulation of endocytosis; positive regulation of neurotransmitter secretion; positive regulation of peptidyl-serine phosphorylation; positive regulation of receptor recycling; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of synaptic transmission; protein destabilization; receptor internalization; regulation of acyl-CoA biosynthetic process; regulation of dopamine secretion; regulation of excitatory postsynaptic membrane potential; regulation of glutamate secretion; regulation of locomotion; regulation of long-term neuronal synaptic plasticity; regulation of macrophage activation; regulation of neuron apoptosis; regulation of neuronal synaptic plasticity; regulation of neurotransmitter secretion; response to cocaine; response to drug; response to iron(II) ion; response to lipopolysaccharide; response to magnesium ion; synapse organization and biogenesis; synaptic transmission; synaptic transmission, dopaminergic; synaptic vesicle endocytosis; synaptic vesicle transport