Rabbit ALPHA SYNUCLEIN Polyclonal Antibody | anti-Snca antibody

RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129)

Cat. Num. AAA222961

• Reactivity: Bovine (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence)
• Applications: Western Blot
• Synonyms: ALPHA SYNUCLEIN; Polyclonal Antibody; RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129); anti-Snca antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 140

• Applicable Applications for anti-Snca antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 15kDa in rat cortex lysate.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 50% Glycerol; 0.01% Bovine Serum Albumin
• Immunogen: Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein.; Buffer Solution
• Antiserum Preparation: Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-Snca antibody

MBS222961 specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.

NCBI and Uniprot Product Information

• NCBI GI #: 9507125
• NCBI GeneID: 29219
• NCBI Accession #: NP_062042.1
• NCBI GenBank Nucleotide #: NM_019169.2
• UniProt Accession #: P37377; P37378; Q53YM9
• Molecular Weight: 4,354 Da
• NCBI Official Full Name: alpha-synuclein
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: Snca
• NCBI Protein Information: alpha-synuclein
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: Snca
• UniProt Entry Name: SYUA_RAT

NCBI Description

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Cellular Component: Golgi apparatus; nuclear outer membrane; cytoplasmic vesicle membrane; mitochondrion; rough endoplasmic reticulum; intracellular membrane-bound organelle; cell; fibril; terminal button; inclusion body; cell cortex; cytosol; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; cytoskeleton; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; synapse; nerve terminal; nucleus

Molecular Function: identical protein binding; histone binding; zinc ion binding; kinesin binding; ferrous iron binding; magnesium ion binding; calcium ion binding; oxidoreductase activity; Hsp70 protein binding; phospholipase binding; enzyme binding; phospholipid binding; fatty acid binding; alpha-tubulin binding; protein domain specific binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; arachidonic acid binding; dynein binding; protein binding; copper ion binding; tau protein binding

Biological Process: regulation of acyl-CoA biosynthetic process; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; dopamine metabolic process; calcium ion homeostasis; response to magnesium ion; regulation of neuron apoptosis; synapse organization and biogenesis; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; negative regulation of apoptosis; regulation of neuronal synaptic plasticity; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; negative regulation of serotonin uptake; mitochondrial membrane organization and biogenesis; negative regulation of norepinephrine uptake; microglial cell activation; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of synaptic transmission; negative regulation of histone acetylation; negative regulation of dopamine uptake; synaptic transmission; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; aging; caspase activation; neutral lipid metabolic process; protein destabilization; regulation of glutamate secretion; regulation of macrophage activation; negative regulation of microtubule polymerization; synaptic vesicle transport; response to cocaine; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential