Rabbit anti-Human, Mouse GCLC Polyclonal Antibody | anti-GCLC antibody

GCLC, ID (GCLC, GLCL, GLCLC, Glutamate-cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase) (PE)

Cat. Num. AAA6301642

• Gene Names: GCLC; GCL; GCS; GLCL; GLCLC
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCLC; Polyclonal Antibody; ID (GCLC; GLCL; GLCLC; Glutamate-cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase) (PE); Glutamate--cysteine ligase catalytic subunit; Gamma-glutamylcysteine synthetase; anti-GCLC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GCLC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-315 amino acids from the Central region of human GCLC.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GCLC Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).)

Western Blot (WB)

(GCLC Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).)

Related Product Information for anti-GCLC antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Product Categories/Family for anti-GCLC antibody

Antibodies; Amino Acids

References

Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010). Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010): Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010). Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010). Wang, X., et al. PLoS ONE 5 (8), E11934 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557625
• NCBI GeneID: 2729
• NCBI Accession #: NP_001489.1
• NCBI GenBank Nucleotide #: NM_001498.3
• UniProt Accession #: P48506; Q14399; Q14TF0
• Molecular Weight: 72,766 Da
• NCBI Official Full Name: glutamate--cysteine ligase catalytic subunit isoform a
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, catalytic subunit
• NCBI Official Symbol: GCLC
• NCBI Official Synonym Symbols: GCL; GCS; GLCL; GLCLC
• NCBI Protein Information: glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; OTTHUMP00000217803; gamma-glutamylcysteine synthetase
• UniProt Protein Name: Glutamate--cysteine ligase catalytic subunit
• Protein Family: Glutamate--cysteine ligase
• UniProt Gene Name: GCLC
• UniProt Synonym Gene Names: GLCL; GLCLC
• UniProt Entry Name: GSH1_HUMAN

Uniprot Description

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: EC 6.3.2.2; Other Amino Acids Metabolism - glutathione; Ligase

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; ADP binding; coenzyme binding; ATP binding

Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; response to heat; sulfur amino acid metabolic process; cell redox homeostasis; xenobiotic metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; response to oxidative stress; regulation of blood vessel size; negative regulation of transcription, DNA-dependent; regulation of mitochondrial depolarization; negative regulation of apoptosis

Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To