Goat anti-Human ALOX15 Polyclonal Antibody | anti-ALOX15 antibody

ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)

Cat. Num. AAA622181

• Gene Names: ALOX15; 15LOX-1; 15-LOX-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: ALOX15; Polyclonal Antibody; ALOX15 (Arachidonate 15-lipoxygenase; 15-LOX-1; 15-LOX2); Anti -ALOX15 (Arachidonate 15-lipoxygenase; anti-ALOX15 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ALOX15.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ALOX15 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA: 1:32,000.; Western Blot: 0.2-0.5µg/ml. Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa-HYKTDVAVKDDPE.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(A4776-31 (0.2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-ALOX15 antibody

Antibodies; Abs to Leukotrienes

NCBI and Uniprot Product Information

• NCBI GI #: 40316937
• NCBI GeneID: 246
• NCBI Accession #: NP_001131.3
• NCBI GenBank Nucleotide #: NM_001140.3
• UniProt Accession #: P16050; Q8N6R7; Q99657; A8K2P4; Q12938
• Molecular Weight: 74,804 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase
• NCBI Official Symbol: ALOX15
• NCBI Official Synonym Symbols: 15LOX-1; 15-LOX-1
• NCBI Protein Information: arachidonate 15-lipoxygenase; 15-LOX; 15-lipooxygenase-1; OTTHUMP00000115936; arachidonate omega-6 lipoxygenase
• UniProt Protein Name: Arachidonate 15-lipoxygenase
• UniProt Gene Name: ALOX15
• UniProt Synonym Gene Names: LOG15
• UniProt Entry Name: LOX15_HUMAN

Uniprot Description

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - linoleic acid; Oxidoreductase; Motility/polarity/chemotaxis; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; EC 1.13.11.31

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; lipoxygenase pathway; positive regulation of actin filament polymerization; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance