Western Blot (WB)
(Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using MBS619118.)
Ajuba Polyclonal Antibody | anti-Ajuba antibody
Anti - Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba)
Gene Names
JUB; Ajuba; MGC15563; JUB
Reactivity
Bovine, Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Flow Cytometry
Purity
Purified by Protein A and peptide affinity chromatography.
Synonyms
Ajuba; Polyclonal Antibody; Anti - Ajuba (JUB; Ajuba Homolog (Xenopus laevis); MGC15563; Protein Ajuba); Ajuba (JUB; anti-Ajuba antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Bovine, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total Ajuba protein. Species crossreactivity
Purity/Purification
Purified by Protein A and peptide affinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Sequence Length
538
Applicable Applications for anti-Ajuba antibody
Western Blot, Immunoprecipitation, Flow Cytometry
Application Notes
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Dilution: Flow Cytometry: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Dilution: Flow Cytometry: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide corresponding to human Ajuba.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using MBS619118.)
Western Blot (WB)
(Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using MBS619118.)
Western Blot (WB)
(Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using MBS619118.)
Western Blot (WB)
(Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using MBS619118.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of COS cells, using MBS619118 (blue) compared to a non-specifc negative control antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of COS cells, using MBS619118 (blue) compared to a non-specifc negative control antibody (red).)
Related Product Information for anti-Ajuba antibody
Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4).
Product Categories/Family for anti-Ajuba antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,934 Da
NCBI Official Full Name
ajuba
NCBI Official Synonym Full Names
jub, ajuba homolog (Xenopus laevis)
NCBI Official Symbol
JUB
NCBI Official Synonym Symbols
Ajuba; MGC15563; JUB
NCBI Protein Information
protein ajuba
UniProt Protein Name
Protein ajuba
Protein Family
UniProt Gene Name
JUB
UniProt Entry Name
JUB_HUMAN
Similar Products
Product Notes
The Ajuba jub (Catalog #AAA619118) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba) reacts with Bovine, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ajuba can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunoprecipitation, Flow Cytometry. Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Dilution: Flow Cytometry: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Ajuba jub for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ajuba, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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