Rabbit Vaccinia Related Kinase 3 Polyclonal Antibody | anti-VRK3 antibody

Vaccinia Related Kinase 3 (VRK3)

Cat. Num. AAA620008

• Gene Names: VRK3; VRK3
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by protein A and peptide affinity chromatography.
• Synonyms: Vaccinia Related Kinase 3; Polyclonal Antibody; Vaccinia Related Kinase 3 (VRK3); Anti -Vaccinia Related Kinase 3 (VRK3); anti-VRK3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total VRK3 protein.
• Purity/Purification: Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-VRK3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to human VRK3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from Caki, C2C12 and COS cells using MBS620008.)

Related Product Information for anti-VRK3 antibody

The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses ERK activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation.

Product Categories/Family for anti-VRK3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 31324166
• NCBI GeneID: 51231
• UniProt Accession #: Q8IV63; Q502Y2; Q9P2V8; A8KA53
• Molecular Weight: 52,881 Da
• NCBI Official Full Name: vaccinia virus B1R kinase related kinase 3
• NCBI Official Synonym Full Names: vaccinia related kinase 3
• NCBI Official Symbol: VRK3
• NCBI Official Synonym Symbols: VRK3
• NCBI Protein Information: serine/threonine-protein kinase VRK3; vaccinia-related kinase 3
• UniProt Protein Name: Inactive serine/threonine-protein kinase VRK3
• Protein Family: Inactive serine/threonine-protein kinase
• UniProt Gene Name: VRK3
• UniProt Entry Name: VRK3_HUMAN

NCBI Description

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq]

Uniprot Description

VRK3: Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. 2 human isoforms produced by alternative splicing.

Protein type: Protein kinase, CK1; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CK1 group; VRK family

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; regulation of cell shape; peptidyl-serine phosphorylation; Wnt receptor signaling pathway; endocytosis