Mouse anti-Human, Rat LPP Monoclonal Antibody | anti-LPP antibody

Anti-LPP Mouse mAb

Cat. Num. AAA475784

• Reactivity: Human, Rat
• Applications: Western Blot
• Synonyms: LPP; Monoclonal Antibody; Anti-LPP Mouse mAb; DKFZp779O0231; FLJ30652; FLJ41512; LIM domain containing preferred translocation partner in lipoma; LIM domain-containing preferred translocation partner in lipoma; LIM protein; Lipoma preferred partner; Lipoma-preferred partner; lpp; LPP_HUMAN; anti-LPP antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6F6-C1-B6
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 612

• Applicable Applications for anti-LPP antibody: Western Blot (WB)
• Immunogen: Purified recombinant fragment of human LPP expressed in E Coli.
• Sequence Similarities: Contains 3 LIM zinc-binding domains.
• Subunit Structure: Interacts with VASP, with PDZ domains of SCRIB and with ACTN1/alpha-actinin.
• Subcellular Location: Cell membrane

Western Blot (WB)

(Western blot detection of LPP in A549, MCF7, C6 and Hela cell lysates using LPP mouse mAb (1:5000 diluted).Predicted band size:66KDa.Observed band size:75KDa.)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis of COS cells using LPP mouse mAb (green). Red)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis of Hela cells using LPP mouse mAb (green). Red)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human vessels tissues using LPP mouse mAb.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human small intestine using LPP mouse mAb with DAB staining.)

Related Product Information for anti-LPP antibody

Entrez Summary: LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP, TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxy terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts, and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentially translocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.
UniPort Summary Function: May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 264681524
• NCBI GeneID: 4026
• NCBI Accession #: NP_001161143.1
• NCBI GenBank Nucleotide #: NM_001167671.2
• UniProt Accession #: Q93052; Q8NFX5; A1L4L6; D3DNV6
• Molecular Weight: 75kDa
• NCBI Official Full Name: lipoma-preferred partner isoform a
• NCBI Official Synonym Full Names: LIM domain containing preferred translocation partner in lipoma
• NCBI Official Symbol: LPP
• NCBI Protein Information: lipoma-preferred partner
• UniProt Protein Name: Lipoma-preferred partner
• Protein Family: LPP20 lipoprotein
• UniProt Gene Name: LPP

NCBI Description

This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

LPP: a LIM domain-containing that may function in focal adhesion and nuclear targeting.

Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q27.3-q28

Cellular Component: cytoplasm; focal adhesion

Molecular Function: protein binding

Disease: Leukemia, Acute Myeloid